Render Target: STATIC
Render Timestamp: 2025-01-21T04:19:25.193Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-01 15:26:26.015
Product last modified at: 2025-01-01T09:06:53.755Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ALKBH7 Antibody #15093

Filter:
  • WB
Western Blotting Image 1: ALKBH7 Antibody
Western blot analysis of extracts from U266, K-562, and Raji cells using ALKBH7 Antibody.

To Purchase # 15093

Cat. #

Size

15093S
100 µl

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 25
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ALKBH7 Antibody recognizes endogenous levels of total ALKBH7 protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val34 of human ALKBH7 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The AlkB alkylation repair homolog 7 (ALKBH7, ABH7) is a member of the alkylated DNA repair homolog family that is responsible for repair of DNA damage induced by oxidation and alkylation (1). ALKBH7 is a nuclear encoded protein that contains an amino-terminal mitochondrial targeting sequence that directs import of ALKBH7 to the mitochondria (2). Unlike other Alkb family members, the ALKBH7 protein lacks a functional nucleotide recognition lid essential for nucleobase-binding, which abrogates any DNA or RNA repair capability (3). In response to DNA damage, mitochondrial ALKBH7 triggers the collapse of the mitochondrial membrane potential. The resultant loss of mitochondrial function leads to depletion of cellular energy and programmed cell death (2). Research studies indicate that ALKBH7 knockdown cells are resistant to apoptotic cell death induced by oxidizing and alkylating agents, which suggests that ALKBH7 may play a novel function in promoting cell death (2). Indeed, ALKBH7 has been identified as a key regulator of the alkylation or oxidizing DNA damaged induced necroptosis pathway (2).
For Research Use Only. Not For Use In Diagnostic Procedures.
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