Phospho-SLP-76 (Ser376) (E3G9U) XP® Rabbit mAb (Pacific Blue™ Conjugate) #15642
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Following TCR ligation, SLP-76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase 1 (HPK1) (6,7). This phosphorylation induces interaction with 14-3-3ε, which leads to the disassembly of TCR signaling complexes and downregulation of TCR signaling (6-8).
- Clements, J.L. (2003) Immunol Rev 191, 211-9.
- Bubeck Wardenburg, J. et al. (1998) Immunity 9, 607-16.
- Bunnell, S.C. et al. (2000) J Biol Chem 275, 2219-30.
- Liu, S.K. et al. (1999) Curr Biol 9, 67-75.
- Clements, J.L. et al. (1998) J Immunol 161, 3880-9.
- Shui, J.W. et al. (2007) Nat Immunol 8, 84-91.
- Di Bartolo, V. et al. (2007) J Exp Med 204, 681-91.
- Lasserre, R. et al. (2011) J Cell Biol 195, 839-53.
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