Bcl-11B (D6F1) XP® Rabbit mAb (BSA and Azide Free) #16148
- WB
- IF
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120-130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Bcl-11B (D6F1) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total Bcl-11B protein.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein.
Background
Bcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C2H2 type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the BCL11B gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2nd and 3rd zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9).
- Avram, D. et al. (2000) J Biol Chem 275, 10315-22.
- Liu, P. et al. (2010) Immunol Rev 238, 138-49.
- Kominami, R. (2012) Proc Jpn Acad Ser B Phys Biol Sci 88, 72-87.
- Wakabayashi, Y. et al. (2003) Biochem Biophys Res Commun 301, 598-603.
- Cismasiu, V.B. et al. (2005) Oncogene 24, 6753-64.
- Topark-Ngarm, A. et al. (2006) J Biol Chem 281, 32272-83.
- Marban, C. et al. (2007) EMBO J 26, 412-23.
- Gutierrez, A. et al. (2011) Blood 118, 4169-73.
- Obata, M. et al. (2012) Cell Signal 24, 1047-52.
Limited Uses
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