BATF3 (E3K5H) Rabbit mAb #16484
- WB
- IP
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #62132.
Protocol
Specificity / Sensitivity
BATF3 (E3K5H) Rabbit mAb recognizes endogenous levels of total human BATF3 protein. This clone exhibits reactivity by western blot in cell lines transfected with a construct expressing Myc/DDK-tagged mouse BATF3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BATF3 protein.
Background
BATF3 (Basic leucine zipper transcriptional factor ATF-like 3, p21SNFT) is a member of the basic leucine zipper family (1,2,5) which dimerizes with Jun proteins and represses activator protein-1 (AP-1) activity (1). BATF3 is required for development of conventional type 1 dendritic cells (cDC1s), which cross-present antigens in the context of MHC class I to naïve CD8+ T cells, priming them to become activated cytotoxic CD8+ T cells (2-4).
Limited Uses
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