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Product last modified at: 2025-01-08T21:45:11.414Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

G4S Linker (E7O2V) Rabbit mAb (Biotinylated) #17621

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Flow Cytometry Image 1: G4S Linker (E7O2V) Rabbit mAb (Biotinylated)
Flow cytometric analysis of live Jurkat cells (blue, negative) or Jurkat cells engineered to stably express an scFv-based Anti-CD19 CAR containing a G4S linker (green, positive) using G4S Linker (E7O2V) Rabbit mAb (Biotinylated) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Biotinylated) #4096 (dashed lines). Anti-biotin (D5A7) Rabbit mAb (PE Conjugate) #39681 was used as a secondary antibody. Engineered cell line was provided by the Lohmueller Lab, University of Pittsburgh.

To Purchase # 17621

Cat. # Size Qty. Price Ships
17621S 100 µl
$493
17621L 300 µl
$1,404

Supporting Data

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Rabbit IgG
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645.

Product Usage Information

Application Dilution
Flow Cytometry (Live) 1:50

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

G4S Linker (E7O2V) Rabbit mAb (Biotinylated) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.

Background

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or 20-mer (G4S)4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).
For Research Use Only. Not For Use In Diagnostic Procedures.
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