ATL3 (F5T5J) Rabbit mAb #19901
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
ATL3 (F5T5J) Rabbit mAb recognizes endogenous levels of total ATL3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly519 of human ATL3 protein.
Background
Atlastin-3 (ATL3) is a dynamin-like, integral membrane GTPase that is localized to the endoplasmic reticulum (ER) membrane and critical for ER morphogenesis and tubular network biogenesis (1-3). ATL3 also functions as a cargo receptor for ER-phagy and promotes tubular ER degradation by specifically binding to GABARAP subfamily proteins. This ATL3-GABARAP interaction is essential for ATL3 to function in ER-phagy (4). Mutant ATL3 proteins are associated with hereditary sensory and autonomic neuropathies, illustrating the dependence of long-term sensory neuronal maintenance on the structural organization of the ER (5,6). Zika and coronavirus have been shown to modulate ER-phagy to drive viral replication by hijacking ATL3 and other ER-phagy receptors (7,8).
- Rismanchi, N. et al. (2008) Hum Mol Genet 17, 1591-604.
- Hu, J. et al. (2009) Cell 138, 549-61.
- Wang, S. et al. (2016) Elife 5, e18605. doi: 10.7554/eLife.18605.
- Chen, Q. et al. (2019) Curr Biol 29, 846-855.e6.
- Kornak, U. et al. (2014) Brain 137, 683-92.
- Krols, M. et al. (2018) Cell Rep 23, 2026-2038.
- Monel, B. et al. (2019) J Virol 93, e01047-19. doi: 10.1128/JVI.01047-19.
- Tan, X. et al. (2023) Cell Rep 42, 112286.
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