MAGE-C2/CT10 (E7B8D) XP® Rabbit mAb #20058
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:4000 - 1:16000 |
Storage
For a carrier free (BSA and azide free) version of this product see product #16675.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Melanoma-associated antigen-C2/Cancer testis antigen 10 (MAGE-C2/CT10) belongs to the type I MAGE family of proteins. In normal human tissues, MAGE-C2/CT10 expression is nuclear and largely restricted to germline cells of the testis (4). At the molecular level, research studies have demonstrated that MAGE-C2/CT10 physically interacts with and enhances activity of the TRIM28 E3 RING ubiquitin ligase (5,6). MAGE-C2/CT10 has also been shown to interact with and regulate the activity of SCF CRL E3 ubiquitin ligase complexes toward substrates that control cell cycle progression, suggesting a role for MAGE-C2/CT10 in malignant transformation (7). Indeed, MAGE-C2/CT10 is aberrantly expressed in testicular germ cell tumors, prostate carcinoma, hepatocellular carcinoma, and melanoma (8-11). Furthermore, MAGE-C2/CT10 is immunogenic in some tumor types and as a result, there is interest in targeting antigenic epitopes presented in the context of class I MHC alleles using engineered TCRs (12,13).
- Caballero, O.L. and Chen, Y.T. (2009) Cancer Sci 100, 2014-21.
- De Smet, C. et al. (1999) Mol Cell Biol 19, 7327-35.
- Gjerstorff, M.F. et al. (2015) Oncotarget 6, 15772-87.
- Zhuang, R. et al. (2006) Cancer Immun 6, 7.
- Doyle, J.M. et al. (2010) Mol Cell 39, 963-74.
- Yang, B. et al. (2007) Cancer Res 67, 9954-62.
- Hao, J. et al. (2015) Oncotarget 6, 42028-39.
- Bode, P.K. et al. (2011) Mod Pathol 24, 829-35.
- von Boehmer, L. et al. (2011) PLoS One 6, e21366.
- Riener, M.O. et al. (2009) Int J Cancer 124, 352-7.
- Curioni-Fontecedro, A. et al. (2011) PLoS One 6, e21418.
- Ma, W. et al. (2011) Int J Cancer 129, 2427-34.
- Straetemans, T. et al. (2012) Clin Dev Immunol 2012, 586314.
Limited Uses
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