IRP1 (D6S4J) Rabbit mAb #20272
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 98 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
IRP1 is a bifunctional protein. In iron replete cells, IRP1 is associated with a 4Fe-4S cluster that keeps IRP1 in a closed conformation, and functions as a cytosolic aconitase catalyzing the conversion of citrate into iso-citrate. In iron deficient cells, IRP1 loses the 4Fe-4S cluster and adopts an open structure with IRE-binding activity (7).
- Rouault, T.A. (2006) Nat Chem Biol 2, 406-14.
- Wang, J. and Pantopoulos, K. (2011) Biochem J 434, 365-81.
- Pantopoulos, K. et al. (2012) Biochemistry 51, 5705-24.
- Haro, K.J. et al. (2012) PLoS One 7, e48841.
- Wang, W. et al. (2014) Cancer Res 74, 497-507.
- Jeong, S.M. et al. (2015) Oncogene 34, 2115-24.
- Anderson, C.P. et al. (2012) Biochim Biophys Acta 1823, 1468-83.
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