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Render Timestamp: 2025-01-20T13:16:53.434Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-20 06:15:38.154
Product last modified at: 2025-01-01T09:07:07.911Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

PP2A A Subunit Antibody #2039

Filter:
  • WB
  • IF
  • F
Western Blotting Image 1: PP2A A Subunit Antibody
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using PP2A A Subunit Antibody.

To Purchase # 2039

Cat. #

Size

2039S
100 µl

Supporting Data

REACTIVITY H M R Mk Dm
SENSITIVITY Endogenous
MW (kDa) 62
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Dm-D. melanogaster 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:50
Flow Cytometry (Fixed/Permeabilized) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

This antibody detects endogenous levels of PP2A A subunit, alpha isoform. The antibody does not cross-react with other PP2A subunits.

Species Reactivity:

Human, Mouse, Rat, Monkey, D. melanogaster

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human PP2A A subunit. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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