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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ENPP1 (L520) Antibody #2061

Filter:
  • WB
  • IP
Western Blotting Image 1: ENPP1 (L520) Antibody
Western blot analysis of extracts from rat KNRK and human HepG2 cells using ENPP1 (L520) Antibody.

To Purchase # 2061

Cat. # Size Qty. Price
2061S 100 µl
$306

Supporting Data

REACTIVITY H R
SENSITIVITY Endogenous
MW (kDa) 140 (Human), 110 (Rodent)
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ENPP1 (L520) Antibody detects endogenous levels of total ENPP1 protein.

Species Reactivity:

Human, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu520 of human ENPP1. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).
For Research Use Only. Not For Use In Diagnostic Procedures.
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