Render Target: STATIC
Render Timestamp: 2025-01-29T11:17:37.103Z
Commit: 8d9f38232df81570bbc23eaa560b31cb39dd8776
XML generation date: 2024-09-30 01:58:39.524
Product last modified at: 2025-01-01T09:05:19.221Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

B7-H6 (E9Q7L) Rabbit mAb #20617

Filter:
  • IP
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:200
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    B7-H6 (E9Q7L) Rabbit mAb recognizes endogenous levels of total B7-H6 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro185 of human B7-H6 protein.

    Background

    B7-H6 (NCR3LG1) is a member of the B7 family of proteins that provide costimulatory signals for regulating T cell responses (1-3). B7-H6 is a ligand for the natural killer (NK)-cell–activating receptor NKp30, which triggers anti-tumor NK cell cytotoxicity and cytokine secretion (1). Both forms of B7-H6, cell surface B7-H6 and soluble B7-H6 (sB7-H6), are detected in primary tumors and the tumor microenvironment, but rarely in normal tissues (1-5). Cell surface B7-H6 associates with cell damage, and triggers innate immunity when it binds to NKp30 on NK cells (3,5). Soluble B7-H6 can decrease NKp30 expression on NK cells and induce NK cell dysfunction (4,5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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