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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

eIF2α (L57A5) Mouse mAb #2103

Filter:
  • WB
  • IHC
Western Blotting Image 1: eIF2α (L57A5) Mouse mAb
Western blot analysis of extracts from various cell types using eIF2α (L57A5) Mouse mAb.

To Purchase # 2103

Cat. # Size Qty. Price Ships
2103T 20 µl
$166
2103S 100 µl
$336

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 38
Source/Isotype Mouse IgG2b
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #68433.

Protocol

Specificity / Sensitivity

eIF2α (L57A5) Mouse mAb detects endogenous levels of total eIF2α protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a purified recombinant protein fragment representing sequence in the central region of human eIF2α.

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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