Myc-Tag (9B11) Mouse mAb #2276
- WB
- IP
- IHC
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a kappa |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:250 |
| Immunohistochemistry (Paraffin) | 1:1500 - 1:6000 |
| Immunofluorescence (Immunocytochemistry) | 1:1000 - 1:2000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:500 - 1:2000 |
| Chromatin IP | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #58730.
Protocol
Specificity / Sensitivity
The antibody may cross-react with c-myc protein. The antibody may weakly cross-react with a protein of unknown origin ~90kDa.
Species Reactivity:
Source / Purification
Background
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
Limited Uses
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