Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
Trial Size Available Flag

Myc-Tag (9B11) Mouse mAb #2276

Filter:
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or transiently transfected with a construct expressing Myc/DDK-tagged SARS-CoV-2 RNA-dependent RNA polymerase protein (lane 2) using Myc-Tag (9B11) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 2276

Cat. # Size Qty. Price
2276T 20 µl
$143
2276S 100 µl
$336

Product Specifications

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
Source/Isotype Mouse IgG2a kappa
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ChIP-Chromatin Immunoprecipitation 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:250
Immunohistochemistry (Paraffin) 1:1500 - 1:6000
Immunofluorescence (Immunocytochemistry) 1:1000 - 1:2000
Flow Cytometry (Fixed/Permeabilized) 1:500 - 1:2000
Chromatin IP 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #58730.

Protocol

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb detects exogenously expressed proteins containing the Myc epitope tag. This antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody may cross-react with c-myc protein. This antibody may weakly cross-react with a protein of unknown origin ~90kDa.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

Alternate Names

myc

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.