CD16 (3G8) Mouse mAb (PE-Cy7® Conjugate) #23290
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:20 |
| Flow Cytometry (Live) | 1:20 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
CD16A is expressed by NK cells, macrophages, and a subset of monocytes, while CD16B is expressed by neutrophils (4). CD16 is commonly used in combination with CD56 to characterize NK cells, with CD16 identifying NK cells capable of cytotoxicity (5).The 3G8 antibody is widely used as a marker of CD16 expression on the cell types mentioned above (6).
- Maenaka, K. et al. (2001) J. Biol. Chem. 276, 44898-44904.
- Fridman, W. H. et al. (1992) Immunol. Rev. 125, 49-76.
- Pignata, C. et al. (1993) J. Immunol. 151, 6794-6800.
- Pincetic, A. et al. (2014) Nat Immunol 15, 707-16.
- Nagler, A. et al. (1989) J Immunol 143, 3183-91.
- Fleit, H.B. et al. (1982) Proc Natl Acad Sci U S A 79, 3275-9.
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