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DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) #2368

Filter:
  • WB
  • IP
  • F
Western blot analysis of extracts from COS or 293T cells, mock transfected (lane 1) or expressing DYKDDDDK-tagged p73 (lane2), DYKDDDDK-tagged FKHRL1 (lane 3) or truncated DYKDDDDK-tagged huntingtin (lane 4), using DYKDDDDK Tag Antibody.

To Purchase # 2368

Cat. # Size Qty. Price
2368T 20 µl
$173
2368S 100 µl
$306

Product Specifications

REACTIVITY All
SENSITIVITY Transfected Only
MW (kDa)
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • All-All Species Expected 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Flow Cytometry (Fixed/Permeabilized) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) detects exogenously expressed DYKDDDDK proteins in cells. This antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody) fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.

Species Reactivity:

All Species Expected

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).

Alternate Names

DDK; Flag; Flag tag; Flag-tag

For Research Use Only. Not for Use in Diagnostic Procedures.
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