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Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-11-18 16:03:12.855
Product last modified at: 2025-01-15T19:00:09.886Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CCL18 (F9S6V) Rabbit mAb #23957

Filter:
  • WB
  • F
Western Blotting Image 1: CCL18 (F9S6V) Rabbit mAb
Western blot analysis of U-937 and HeLa cells, untreated (-) or treated (+) as indicated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (10 ng/mL, 48 hr), followed by Human IL-4 Recombinant Protein #74319 (hIL-4; 45 ng/mL, 3 days) and Brefeldin A #9972 (300 ng/mL, final 24 hr), using CCL18 (F9S6V) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). CCL18 is induced by TPA + hIL-4 treatment as expected based on PMID: 25502147.

To Purchase # 23957

Cat. # Size Qty. Price Ships
23957T 20 µl
$155
23957S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 9
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CCL18 (F9S6V) Rabbit mAb recognizes endogenous levels of total CCL18 protein. This antibody does not cross-react with CCL3/MIP-1α or CCL4 proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCL18 protein.

Background

C-C motif chemokine ligand 18 (CCL18, PARC, MIP-4, AMAC-1, DC-CK1) is a member of the CC chemokine family involved in signaling and coordinating immune responses (1-3). CCL18 is a secreted protein, primarily expressed by macrophages, monocytes, and dendritic cells (1-3). Th2-associated anti-inflammatory cytokines such as IL-4, IL-13, and IL-10 can polarize macrophages into an M2 phenotype, which induces CCL18 expression (3,4). In cancer, tumor-associated macrophages (TAMs) may secrete large amounts of CCL18, causing an immunosuppressive tumor microenvironment, which can lead to cancer immune evasion (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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