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Render Timestamp: 2025-01-22T10:24:51.977Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-26 13:05:09.789
Product last modified at: 2025-01-01T09:05:27.120Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD3 (17A2) Rat mAb (APC Conjugate) #24265

Filter:
  • IF
  • F
Immunofluorescence Image 1: CD3 (17A2) Rat mAb (APC Conjugate)
Confocal immunofluorescent analysis of fixed frozen mouse spleen, labeled with CD3 (17A2) Rat mAb (APC Conjugate) #24265 (left, red) and co-labeled with HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (right, green) and DAPI #4083 (right, blue).

To Purchase # 24265

Cat. #

Size

24265S
100 µg

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rat IgG2b kappa
Application Key:
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to allophycocyanin (APC) and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.

Application Dilution
Immunofluorescence (Frozen) 1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized) 1:40
Flow Cytometry (Live) 1:40

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD3 (17A2) Rat mAb (APC Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. This antibody detects epitopes within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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