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Shc Antibody #2432

Filter:
  • WB
Western Blotting Image 1: Shc Antibody
Western blot analysis of extracts from HepG2 and Jurkat cells, using Shc Antibody.

To Purchase # 2432

Cat. # Size Qty. Price
2432S 100 µl
$306

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 46, 52, 66
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Shc Antibody detects endogenous levels of total Shc protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy-terminal PTB domain of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SHC-transforming protein 1 (SHC1) is an adaptor protein to targets of activated tyrosine kinases. SHC1 is ubiquitously expressed and the gene is expressed in 2 transcripts, resulting in 3 isoforms with distinct functions and subcellular locations (1). The phosphorylated p46 and p52 isoforms couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex. The third isoform, p66, acts as a downstream target of the tumor suppressor p53 and is thereby implicated in oxidative stress response, tumor progression, and cancer cell proliferation (2,3). SHC1 has been identified to have prognostic and diagnostic value in multiple cancers, making it a potential biomarker (4).

Alternate Names

rtkscaffold

Pathways

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For Research Use Only. Not for Use in Diagnostic Procedures.
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