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Product last modified at: 2025-01-10T15:30:12.310Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

eIF4H Antibody #2444

Filter:
  • WB
Western Blotting Image 1: eIF4H Antibody
Western blot analysis of extracts from various cell types using eIF4H Antibody.

To Purchase # 2444

Cat. # Size Qty. Price Ships
2444S 100 µl
$306

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 25, 27
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

eIF4H Antibody detects endogenous levels of total eIF4H protein (both long and short isoforms).

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human eIF4H. Antibodies are purified by protein A and peptide affinity chromatography.

Background

A variety of factors contribute to the initiation of translation. Eukaryotic translation initiation factor 4H (eIF4H) was purified to near homogeneity from rabbit reticulocyte lysate and shown to stimulate translation in an assay deficient in eIF4F and eIF4B (1). eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (1,2). eIF4H was further shown to stimulate the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (3). Interaction between eIF4H and the herpes simplex virus shutoff protein (Vhs) appears to be important for Vhs-mediated degradation of mRNA (4). Deletion of a large region of chromosome 7, including the corresponding eIF4H gene, results in Williams-Beuren Syndrome (WBS), an autosomal dominant disorder that can present with cardiovascular problems, mental retardation and distinctive facial features (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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