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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LAIR-1 (E7X6I) XP® Rabbit mAb (BSA and Azide Free) #25289

Filter:
  • WB
  • IHC
  • IF
  • F
Western Blotting Image 1: LAIR-1 (E7X6I) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from THP-1 and HeLa cells using LAIR-1 (E7X6I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, HeLa cells are low or negative for LAIR-1 expression. Data were generated using the standard formulation of this product.

To Purchase # 25289

Cat. # Size Qty. Price
25289SF 100 µg
$843

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 50
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #60061. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #60061

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

LAIR-1 (E7X6I) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total LAIR-1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LAIR-1 protein.

Background

LAIR-1 is an inhibitory receptor that belongs to the Immunoglobulin superfamily. It has one extracellular Ig-like domain and an intracellular C-terminus with two ITIM (immunoreceptor tyrosine-based inhibitory motif) domains. It is found on peripheral mononuclear cells, including NK, T, and B cells, and is thought to play a negative regulatory role on the cytolytic function of these cells through signaling through collagen ligation (1). LAIR1 has been noted to be upregulated in renal cell carcinoma (2), and may play a role in expansion of Th17 cell populations in collagen-rich environments, such as in graft rejection tissue (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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