Render Target: STATIC
Render Timestamp: 2025-01-24T12:27:01.690Z
Commit: 8d9f38232df81570bbc23eaa560b31cb39dd8776
XML generation date: 2024-09-30 01:54:42.145
Product last modified at: 2025-01-01T09:05:02.195Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SPAR (D9P9T) Rabbit mAb #25823

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 13
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SPAR (D9P9T) Rabbit mAb recognizes endogenous levels of total SPAR protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SPAR protein.

    Background

    Small regulatory Polypeptide of Amino Acid Response (SPAR) is a polypeptide, encoded by the lncRNA LINC00961 gene and is localized in late endosomal and lysosomal membranes. SPAR binds to the lysosomal v-ATPase complex in addition to ragulator, RagA and RagC. Upon amino acid stimulation, this supercomplex activates mTORC1 signaling. However, this process is inhibited by the binding of SPAR to the v-ATPase complex, as its formation prevents mTORC1 recruitment to lysosomes. SPAR downregulation and inactivation is an important focus for promoting muscle regeneration following muscle injury. This downregulation and inactivation promotes mTORC1 activation and results in myofiber maturation. In addition to muscle regeneration, SPAR downregulation and inactivation also promotes stem cell proliferation and differentiation.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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