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PDP - Template Name: Polyclonal Antibody (Isotype Control)
PDP - Template ID: *******afc9daa

Normal Rabbit IgG #2729

Filter:
  • IP
  • ChIP
  • C&T
Immunoprecipitation Image 1: Normal Rabbit IgG
Immunoprecipitation of 4E-BP2 from C2C12 cell extracts using Normal Rabbit IgG #2729 (lane 2) or 4E-BP2 Antibody #2845 (lane 3). Lane 1 is 10% input. Western blot analysis was performed using 4E-BP2 Antibody #2845.

To Purchase # 2729

Cat. # Size Qty. Price Ships
2729S 250 µl
$100 Jan 22

Supporting Data

Concentration 1 mg/ml
SENSITIVITY Endogenous
Source/Isotype Rabbit
Application Key:
  • IP-Immunoprecipitation 
  • ChIP-Chromatin Immunoprecipitation 
  • C&T-CUT & Tag 

Product Information

Product Description

Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific IgG control in chromatin immunoprecipitation using our SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003.

Product Usage Information

Important! Dilute this control antibody to the same concentration (not dilution) as the specific test antibody in the chromatin immunoprecipitation. This is typically 1 μl (1 μg) to 5 μl (5 μg) of control antibody for one immunoprecipitation. Higher background signal may result if an excessive amount of rabbit IgG isotype control is used.

Application Dilution
CUT&Tag 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Source / Purification

The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.

Background

Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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