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Product last modified at: 2025-01-01T09:04:49.567Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Argonaute 2 (C34C6) Rabbit mAb #2897

Filter:
  • WB
  • IP
Western Blotting Image 1: Argonaute 2 (C34C6) Rabbit mAb
Western blot analysis of extracts from various cell types using Argonaute 2 (C34C6) Rabbit mAb.

To Purchase # 2897

Cat. # Size Qty. Price
2897T 20 µl
$145
2897S 100 µl
$339

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 97
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Argonaute 2 (C34C6) Rabbit mAb detects endogenous levels of total Argonaute 2 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro30 of mouse Argonaute 2 protein.

Background

Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. miRNAs are about 21 nucleotides in length and have been implicated in many cellular processes, such as development, differentiation, and stress response (1,2). miRNAs regulate gene expression by modulating mRNA translation or stability (2). miRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family, and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of miRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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