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Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:08:28.930
Product last modified at: 2025-03-24T09:30:27.416Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

PD-L1 (405.9A11) Mouse mAb #29122

Filter:
  • WB
  • IHC
Western Blotting Image 1: PD-L1 (405.9A11) Mouse mAb
Western blot analysis of extracts from various cell lines using PD-L1 (405.9A11) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS-299 cell line source: Dr Abraham Karpas, University of Cambridge.

To Purchase # 29122

Cat. # Size Qty. Price
29122T 20 µl
$180
29122S 100 µl
$436

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 40-50
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #39356.

Protocol

Specificity / Sensitivity

PD-L1 (405.9A11) Mouse mAb recognizes endogenous levels of total PD-L1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PD-L1 protein.

Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor-infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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