Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 Moesin. 80 Ezrin, Radixin. |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody detects endogenous levels of ezrin, radixin and moesin only when phosphorylated at threonine 567, 564 or 558, respectively. This antibody does not cross-react with related phospho-proteins such as merlin or band 4.1.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Xenopus, Dog, C. elegans
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
- Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10.
- Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92.
- Matsui, T. et al. (1998) J Cell Biol 140, 647-57.
- Gautreau, A. et al. (2000) J Cell Biol 150, 193-203.
- Tran Quang, C. et al. (2000) EMBO J 19, 4565-76.
- Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5.
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