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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

ECM Profiling Antibody Sampler Kit #33437

ECM Profiling Antibody Sampler Kit: Image 1
Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP® Rabbit mAb.

To Purchase # 33437

Cat. # Size Qty. Price Ships
33437T 1 Kit
9 x 20 microliters
$762 Jan 22
  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
MMP-1 (E9S9N) Rabbit mAb #5437620 µlWB, IP, IHC, IFH55Rabbit IgG
MMP-2 (D4M2N) Rabbit mAb #4099420 µlWB, IP, IHC, IFH64,72Rabbit IgG
MMP-9 (D6O3H) XP® Rabbit mAb #1366720 µlWB, IHC, FH84, 92Rabbit IgG
MMP-13 (E4W3T) Rabbit mAb #6992620 µlWBH60Rabbit IgG
Fibronectin/FN1 (E5H6X) Rabbit mAb #2683620 µlWB, IP, IHC, IFH300Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb #1435120 µlWBH R60Rabbit IgG
LOX (D8F2K) Rabbit mAb #5813520 µlWBH M54, 56Rabbit IgG
COL11A1 (E6O7R) Rabbit mAb #7045820 µlWBH250Rabbit IgG
COL1A1 (E6A8E) Rabbit mAb #3995220 µlWB, IP, IFH220Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBGoat 

Product Information

Product Description

The ECM Profiling Antibody Sampler Kit provides an economical means of detecting endogenous levels of the specific ECM components using the corresponding antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the ECM Profiling Antibody Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val267 of human MMP-1 protein, Phe542 of human MMP-9 protein, Ser417 of human MMP-3 protein, carboxyl terminal region of human MMP13 protein, Glu320 of human COL11A1 protein, Lys170 of human COL1A1 protein, recombinant protein specific to the carboxy terminus of human MMP-2 protein, recombinant protein specific to the carboxy terminus of human FN1 protein, and a recombinant protein specific to human LOX protein.

Background

The extracellular matrix (ECM) is a cell surface associated three-dimensional macromolecular network composed of collagens, proteoglycans/glycosaminoglycans, elastin, fibronectin, laminins, and several other glycoproteins (1). The network provides a dynamic microenvironment surrounding the cell enabling it to carry on its function. Among the ECM proteins, fibronectin functions as a mediator to bridge distinct ECM components such as collagens, growth factors, as well as cell surface integrins to regulate ECM structural change and initiate signaling pathways (2). During normal development and pathological conditions, the ECM network is highly dynamic and continuously undergoes remodeling marked by the change of the ECM structural components, such as COL1A1, COL11A1, fibronectin, and versican. The matrix-degrading enzyme MMPs such as MMP1, MMP2, and MMP9 are highly involved in this process (4). Additional players in this process are the LOX family members of lysyl oxidase. They catalyze the first step of the covalent cross-linking of ECM proteins, collagens, and elastin, which contributes to ECM stiffness and mechanical properties (5).

Pathways

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