Human Sonic Hedgehog (Shh) Recombinant Protein #34052
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 20.2 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hShh. |
| Activity | The bioactivity of recombinant human Shh was determined in a CCL-226 cell proliferation assay. The ED50 of each lot is less than or equal to 5 μg/mL. |
Source / Purification
Background
- Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
- McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
- Zhang, X.M. et al. (2001) Cell 106, 781-792.
- Adolphe, C. et al. (2004) Development 131, 5009-5019.
- Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
- Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.
Limited Uses
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