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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Drosha (D30F3) Rabbit mAb #3410

Filter:
  • WB
  • ChIP
Western Blotting Image 1: Drosha (D30F3) Rabbit mAb
Western blot analysis of extracts from various cell types using Drosha (D30F3) Rabbit mAb.

To Purchase # 3410

Cat. # Size Qty. Price Ships
3410T 20 µl
$152
3410S 100 µl
$336

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 160
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • ChIP-Chromatin Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

Application Dilution
Western Blotting 1:1000
Chromatin IP 1:25

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Drosha (D30F3) Rabbit mAb detects endogenous levels of total Drosha protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His549 of human Drosha.

Background

Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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