Render Target: STATIC
Render Timestamp: 2024-11-21T12:57:35.745Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-11 14:02:07.338
Product last modified at: 2024-11-12T08:01:01.341Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Notch1 (5B5) Rat mAb #3447

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R B
    SENSITIVITY Endogenous
    MW (kDa) 120, 300
    Source/Isotype Rat IgG2b
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Notch1 (5B5) Rat mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). The antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases.

    Species Reactivity:

    Human, Mouse, Rat, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of Notch1.

    Background

    Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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