Render Target: STATIC
Render Timestamp: 2024-11-22T11:37:50.238Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-05 20:49:39.466
Product last modified at: 2024-09-27T21:15:07.536Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Mouse CD28 Activating (37.51) Hamster mAb (Low Endotoxin, Azide-free) #34596

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Hamster (syrian) IgG
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    Endotoxin Less than or equal to 0.01 EU/μg, as determined by the LaL assay.

    Formulation

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, Low Endotoxin, Azide-free.

    Storage

    Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Mouse CD28 Activating (37.51) Hamster mAb (Low Endotoxin, Azide-free) recognizes endogenous levels of CD28 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced against mouse T cell lymphoma EL4 cells and purified via affinity chromatography.

    Background

    CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1,2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6,7). Several CD28 isoforms are produced by alternative splicing (8).
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