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Render Timestamp: 2024-11-21T14:08:22.823Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-07-11 16:01:08.149
Product last modified at: 2024-09-27T23:30:07.896Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Mouse CD3ε Activating (145-2C11) Hamster mAb (Low Endotoxin, Azide-free)

Mouse CD3ε Activating (145-2C11) Hamster mAb (Low Endotoxin, Azide-free) #35425

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Hamster (armenian) IgG
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    Endotoxin Less than or equal to 0.01 EU/μg, as determined by the LaL assay.

    Formulation

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, Low Endotoxin, Azide-free.

    Storage

    Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Mouse CD3ε Activating (145-2C11) Hamster mAb (Low Endotoxin, Azide-free) recognizes endogenous levels of CD3ε protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced against mouse BM10-37 cytotoxic T cells and purified via affinity chromatography.

    Background

    When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).

    Database Links

    UniProt ID: P22646 , P11942


    Entrez-Gene Id: 12501 , 12502


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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