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Product last modified at: 2025-03-08T09:00:10.352Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD14 (61D3) Mouse mAb (APC Conjugate) #36377

Filter:
  • F
Flow Cytometry Image 1: CD14 (61D3) Mouse mAb (APC Conjugate)
Flow cytometric analysis of live human peripheral blood mononuclear cells using CD14 (61D3) Mouse mAb (APC Conjugate) (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (APC Conjugate) #49083 (dashed line). Analysis was performed on cells in the monocyte gate.

To Purchase # 36377

Cat. # Size Qty. Price
36377S 500 µl
100 tests
$276

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometry analysis in human cells.

Product Usage Information

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:20
Flow Cytometry (Live) 1:20

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

CD14 (61D3) Mouse mAb (APC Conjugate) recognizes endogenous levels of total CD14 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Human

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage (1). Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein (2). LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection (3-5).
The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage (6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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