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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ARHGAP29 (F6O5B) Rabbit mAb #37001

Filter:
  • WB
Western Blotting Image 1: ARHGAP29 (F6O5B) Rabbit mAb
Western blot analysis of extracts from various cell lines using ARHGAP29 (F6O5B) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of ARHGAP29 protein in A2780, RAW 264.7, and H-4-II-E cells is consistent with the predicted expression pattern.

To Purchase # 37001

Cat. # Size Qty. Price
37001T 20 µl
$156
37001S 100 µl
$371

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 142
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ARHGAP29 (F6O5B) Rabbit mAb recognizes endogenous levels of total ARHGAP29 protein. By western blot, this antibody detects a band of unknown origin at approximately 10 kDa.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly561 of human ARHGAP29 protein.

Background

Rho GTPase-activating protein 29 (ARHGAP29), also known as PARG1, suppresses Rho family GTPase signaling by promoting GTP hydrolysis and the inactive (GDP-bound) state. Transcription of ARHGAP29 is positively regulated by Yes-associated protein (YAP), destabilizing the actin cytoskeleton and promoting cell migration (1). Upregulated expression of ARHGAP29 is associated with invasion and proliferation in human prostate, breast, and kidney cancer cells, and may serve as a prognostic indicator in several human cancers (1-4). Mutation of the ARHGAP29 gene is associated with nonsyndromic cleft lip and/or palate (NSCL/P) in humans (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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