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PDP - Template Name: ELISA Kit
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  1. Products /
  2. ELISA Kits /
  3. PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit

PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit #37393

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  • ELISA

    Supporting Data

    REACTIVITY Vir
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • Vir-Virus 

    Product Information

    Product Description

    The rapid protocol (RP) PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of SARS-CoV-2 nucleocapsid protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with SARS-CoV-2 nucleocapsid protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of SARS-CoV-2 nucleocapsid protein. Learn more about all of your ELISA kit options here.

    ​​​​​​​*Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The PathScan® RP SARS-CoV-2 Nucleocapsid Protein Sandwich ELISA Kit detects endogenous levels of SARS-CoV-2 nucleocapsid protein, but does not cross-react with nucleocapsid proteins from SARS or MERS coronaviruses, as shown in Figures 1 and 2. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Virus

    Background

    The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7).

    Database Links

    UniProt ID: P0DTC9


    Entrez-Gene Id: 43740575


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    PathScan is a registered trademark of Cell Signaling Technology, Inc.
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