BACH2 (E3N3K) Rabbit mAb #37403
- WB
- IP
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1000 - 1:4000 |
Storage
Protocol
Specificity / Sensitivity
BACH2 (E3N3K) Rabbit mAb recognizes endogenous levels of total BACH2 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of mouse BACH2 protein.
Background
The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders, including type 1 diabetes, celiac disease, Crohn’s disease, and the skin condition known as vitiligo (7-10).
- Oyake, T. et al. (1996) Mol Cell Biol 16, 6083-95.
- Muto, A. et al. (1998) EMBO J 17, 5734-43.
- Muto, A. et al. (2004) Nature 429, 566-71.
- Ochiai, K. et al. (2006) J Biol Chem 281, 38226-34.
- Muto, A. et al. (2010) EMBO J 29, 4048-61.
- Shaffer, A.L. et al. (2002) Immunity 17, 51-62.
- Cooper, J.D. et al. (2008) Nat Genet 40, 1399-401.
- Dubois, P.C. et al. (2010) Nat Genet 42, 295-302.
- Franke, A. et al. (2010) Nat Genet 42, 1118-25.
- Jin, Y. et al. (2012) Nat Genet 44, 676-80.
Limited Uses
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