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Product last modified at: 2025-01-01T09:04:15.625Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ARID4A/RbBP1 (E2T6V) Rabbit mAb #38328

Filter:
  • WB
  • IP
Western Blotting Image 1: ARID4A/RbBP1 (E2T6V) Rabbit mAb
Western blot analysis of extracts from various cell lines using ARID4A/RbBP1 (E2T6V) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb # 6487 (lower). As expected, A-172 cells are low for ARID4A/RbBP1 expression.

To Purchase # 38328

Cat. # Size Qty. Price Ships
38328T 20 µl
$153 Jan 22
38328S 100 µl
$339 Jan 22

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 205
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ARID4A/RbBP1 (E2T6V) Rabbit mAb recognizes endogenous levels of total ARID4A/RbBP1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly813 of human ARID4A/RbBP1 protein.

Background

ARID4A/RbBP1 was originally identified as an RB binding protein, which could repress E2F-mediated transcription through the recruitment of the mSIN3A complex (1-3). Like other AT-rich interacting domain (ARID) containing proteins, ARID4A/RbBP1 functions as a tumor suppressor, and is frequently mutated in colorectal cancer (4-6). ARID4A/RbBP1 and ARID4B/RbBP1L1 are mainly expressed in Sertoli cells in the testes, where they form a complex and serve as coactivators for androgen receptors to regulate spermatogenesis (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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