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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DPEP-1/MDP (E4O6J) XP® Rabbit mAb (BSA and Azide Free) #40632

Filter:
  • WB
  • IHC
  • IF
Western Blotting Image 1: DPEP-1/MDP (E4O6J) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using DPEP-1/MDP (E4O6J) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Absence of signal in extracts from SK-MEL-2 cells is predicted from RNAseq data and confirms the specificity of the antibody for DPEP-1/MDP protein. Data were generated using the standard formulation of this product.

To Purchase # 40632

Cat. # Size Qty. Price
40632SF 100 µg
$843

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 58
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

This product is the carrier free version of product #87223. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #87223

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

DPEP-1/MDP (E4O6J) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total DPEP-1/MDP protein. This antibody does not cross-react with either DPEP-2 or DPEP-3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DPEP-1/MDP protein.

Background

Dipeptidase-1 (DPEP-1, RDP, MDP) is a GPI-linked glycoprotein expressed in the epithelial brush border of the kidney epithelium and exists as a disulfide-linked homodimer (1). Research studies have shown that the enzymatic activity of DPEP-1 is involved in kidney metabolism of glutathione, leukotrienes, and β-lactam antibiotics (2,3). Independent of its enzymatic activity, DPEP-1 has been implicated in pathologic inflammation by acting as an adhesion receptor for neutrophils on endothelial cells within the lung (4). DPEP-1 has also been implicated in human cancer and numerous studies have demonstrated that expression of this enzyme is upregulated in colorectal cancer and promotes metastasis (5-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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