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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Eg5 (4H3-1F12) Mouse mAb #4203

Filter:
  • WB
  • IP
Western Blotting Image 1: Eg5 (4H3-1F12) Mouse mAb
Western blot analysis of extracts form HeLa and 293 cells using Eg5 (4H3-1F12) Mouse mAb.

To Purchase # 4203

Cat. # Size Qty. Price Ships
4203T 20 µl
$152 Jan 22
4203S 100 µl
$336 Jan 22

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 120
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:2000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Eg5 (4H3-1F12) Mouse mAb recognizes endogenous levels of total Eg5 (Kif11) protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant amino-terminal fragment of human Eg5 protein.

Background

Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules (1,2). Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development (3-5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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