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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CCL3/MIP-1α (F3I3P) Rabbit mAb #43710

Filter:
  • WB
Western Blotting Image 1: CCL3/MIP-1α (F3I3P) Rabbit mAb
Western blot analysis of extracts from various human cell lines using CCL3/MIP-1α (F3I3P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of CCL3/MIP-1α protein in HeLa, HT-29, and MCF7 cells is consistent with the predicted expression pattern.

To Purchase # 43710

Cat. # Size Qty. Price Ships
43710T 20 µl
$156
43710S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 10
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CCL3/MIP-1α (F3I3P) Rabbit mAb recognizes endogenous levels of total CCL3/MIP-1α protein. This antibody does not cross-react with CCL4 or CCL18 proteins.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro43 of human CCL3/MIP-1α protein.

Background

Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein-1α (MIP-1α), is a 10 kDa member of the C-C or β family of chemokines that play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 possesses hematopoietic stem cell inhibitory activity, suppressing their proliferation, but has been shown to promote proliferation of more mature progenitors (2-4). CCL3 has been implicated in several forms of leukemia, in which malignant cells may lose responsiveness to CCL3 or produce high levels of CCL3 (2). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (5,6). CCL3 expression is upregulated in the brains of patients with Alzheimer's disease (AD) with dementia, and reduced expression of CCL3 is observed in AD patients resilient to dementia (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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