PathScan® RP β-Amyloid (1-43) Sandwich ELISA Kit #43825
Important Ordering Details
Custom Ordering Details:When ordering five or more kits, please contact us for processing time and pricing.
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Supporting Data
| REACTIVITY | H |
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The rapid protocol (RP) PathScan® RP β-Amyloid (1-43) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of the Aβ-43 isoform of β-amyloid in a reduced assay time of 1.5 hours. This kit also detects to a lesser extent Aβ-42, as shown in Figures 1 and 2. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Aβ-43 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Aβ-43. Learn more about all of your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP β-Amyloid (1-43) Sandwich ELISA Kit detects endogenous levels of the Aβ-43 isoform of β-amyloid peptides, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit also detects to a lesser extent Aβ-42, as shown in Figures 1 and 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human
Background
Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
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