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Render Timestamp:
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4/5/2025, 8:43:51 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:09:46.972
Product last modified at: 2025-03-31T12:45:20.265Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb #45262

Filter:
  • WB
Western Blotting Image 1: Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb
Immunoprecipitation of PRAS40 from untreated HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PRAS40 (D23C7) Rabbit mAb #2691. All lanes are probed with PRAS40 (D23C7) Rabbit mAb as the primary antibody. Secondary antibodies include Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (left panel) and Anti-rabbit IgG, HRP-linked Antibody #7074 (right panel). For the left panel, the bound Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.

To Purchase # 45262

Cat. # Size Qty. Price
45262S 100 µl
$242

Supporting Data

REACTIVITY Rab
SENSITIVITY
MW (kDa)
Source/Isotype Mouse
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • Rab-Rabbit 
  • Related Products

Product Information

Product Usage Information

Recommended Antibody Dilution:

1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.

Species Reactivity:

Rabbit

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

Background

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb as a secondary antibody will eliminate this problem.
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