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Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb #45262

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Supporting Data

REACTIVITY	Rab
SENSITIVITY
MW (kDa)
Source/Isotype	Mouse

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

Rab-Rabbit

Product Information

Product Usage Information

Recommended Antibody Dilution:

1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.

Species Reactivity:

Rabbit

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

Background

Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb as a secondary antibody will eliminate this problem.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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