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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Dynamin-I (3G4B6) Mouse mAb #4565

Filter:
  • WB
  • IP
Western Blotting Image 1: Dynamin-I (3G4B6) Mouse mAb
Western blot analysis of extracts from various cell types using Dynamin-I (3G4B6) Mouse mAb.

To Purchase # 4565

Cat. # Size Qty. Price Ships
4565T 20 µl
$152
4565S 100 µl
$336

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 100
Source/Isotype Mouse IgG2a
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Dynamin-I (3G4B6) Mouse mAb detects endogenous levels of total dynamin-I protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant carboxy-terminal fragment of human dynamin-I.

Background

Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both the endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCγ, PKC and G-proteins. PKC and Src phosphorylate dynamin, and its phosphorylation may regulate the endocytosis of cell surface receptors (2,3).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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