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Product last modified at: 2025-01-01T09:07:05.725Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ARK5 (E4T2A) Rabbit mAb #47677

Filter:
  • WB
  • IHC
Western Blotting Image 1: ARK5 (E4T2A) Rabbit mAb
Western blot analysis of extracts from various cell lines using ARK5 (E4T2A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The lower to negative expression of ARK5 in VCaP cells is consistent with the predicted expression pattern.

To Purchase # 47677

Cat. # Size Qty. Price Ships
47677T 20 µl
$173
47677S 100 µl
$402

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 78
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:125 - 1:500

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ARK5 (E4T2A) Rabbit mAb recognizes endogenous levels of total ARK5 protein.
Non-specific staining was observed in mouse testis by immunohistochemistry.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ARK5 protein.

Background

AMP-activated protein kinases (AMPKs) constitute a serine/threonine protein kinase family, which is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPKα1, AMPKα2, MELK, and SNARK are the catalytic subunits in the family (2). Recently, AMPK-related protein kinase ARK5 was identified, which shares 84% similarity with the sequence of the catalytic domain of SNARK (2). In vitro, Akt phosphorylates ARK5 at Ser600. This phosphorylation activates ARK5, resulting in increased phosphorylation of the SAMS peptide, an AMPK consensus substrate (2). In vivo experiments showed that Akt-activated ARK5 is critical for the survival of cells under glucose starvation (2). Furthermore, studies also linked ARK5 to tumor invasion (3,4). Overexpressed ARK5 leads to a higher tumor growth rate (3). For example, the overexpression of ARK5 in pancreatic tumor cell line PANC-1 significantly elevates its metastasis in the liver (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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