TOFA #47750

Name: TOFA
Price: 86.00 USD
Availability: InStock

Product Information

Product Usage Information

TOFA is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.03 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight	324.5 g/mol
Purity	>98%
Molecular Formula	C₁₉H₃₂O₄
CAS	54857-86-2
Solubility	Soluble in DMSO at 15 mg/mL or ethanol at 9 mg/mL.

Background

The small-molecule TOFA is an allosteric inhibitor of acetyl-CoA carboxylase-α (ACC), which catalyzes carboxylation of acetyl-CoA to malonyl-CoA in cells. It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). Conversion of TOFA to an acetyl-CoA analog (TOFyl-CoA) that cannot be further metabolized to malonyl-CoA creates an allosteric inhibitor of ACC (2). Because many cancer cells require fatty acids for high metabolic needs and rapid proliferation, the reduction of ACC activity is a common target in limiting cancer cell growth. TOFA treatment of lung cancer and colon carcinoma cells blocked fatty acid synthesis, and increased fatty acid oxidation and ketogenesis, inducing apoptosis and cell death (3). Exposure of human pancreatic cancer cells to TOFA resulted in fatty acid depletion, increased caspase-3 activity, and induced cell death (4). TOFA treatment of human renal cell carcinoma inhibited cell growth and arrested cell cycle at G2/M phase, leading to apoptosis (5).

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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