EEA1 (E9Q6G) Mouse mAb #48453
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 170 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
EEA1 (E9Q6G) Mouse mAb recognizes endogenous levels of total EEA1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.
Background
EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).
- Mu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511.
- Christoforidis, S. et al. (1999) Nature 397, 621-625.
- Gaullier, J.M. et al. (1998) Nature 394, 432-433.
- Patki, V. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 7326-7330.
- Lawe, D.C. et al. (2003) Mol. Biol. Cell 14, 2935-2945.
- Simonsen, A. et al. (1998) Nature 394, 494-498.
- McBride, H.M. et al. (1999) Cell 98, 377-386.
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