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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

EEA1 (E9Q6G) Mouse mAb #48453

Filter:
  • WB
  • IP
  • IHC
  • IF
Western Blotting Image 1: EEA1 (E9Q6G) Mouse mAb
Western blot analysis of extracts from T-47D, HeLa, and NIH/3T3 cells using EEA1 (E9Q6G) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 48453

Cat. # Size Qty. Price Ships
48453T 20 µl
$152 Jan 22
48453S 100 µl
$336 Jan 22

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 170
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:50 - 1:250
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:400 - 1:1600
Immunofluorescence (Frozen) 1:50 - 1:200
Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

EEA1 (E9Q6G) Mouse mAb recognizes endogenous levels of total EEA1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.

Background

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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