Render Target: STATIC
Render Timestamp: 2024-12-26T11:35:04.720Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-26 21:22:10.075
Product last modified at: 2024-12-17T18:56:29.247Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Androgen Receptor (D6F11) XP® Rabbit mAb #5153

Filter:
  • WB
  • IP
  • IHC
  • IF
  • F
  • ChIP
  • C&R

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 110
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    • ChIP-Chromatin Immunoprecipitation 
    • C&R-CUT & RUN 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #86652.
    Application Dilution
    Western Blotting 1:2000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Immunocytochemistry) 1:600 - 1:1200
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:800
    Chromatin IP 1:100
    Chromatin IP-seq 1:100
    CUT&RUN 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #23791.

    Protocol

    Specificity / Sensitivity

    Androgen Receptor (D6F11) XP® Rabbit mAb detects endogenous levels of total androgen receptor protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.

    Background

    Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
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