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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

EAPP (1E4) Mouse mAb #5166

Filter:
  • WB
  • IF
Western Blotting Image 1: EAPP (1E4) Mouse mAb
Western blot analysis of extracts from various cell types using EAPP (1E4) Mouse mAb.

To Purchase # 5166

Cat. # Size Qty. Price Ships
5166T 20 µl
$152
5166S 100 µl
$336

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 45
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

EAPP (1E4) Mouse mAb detects endogenous levels of total EAPP protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant amino-terminal fragment of human EAPP protein.

Background

Regulation of the E2F family of transcription factors, primarily through the retinoblastoma protein (pRb), is vital for control of cell proliferation and cell death (reviewed in 1). E2F-associated phosphoprotein (EAPP) was identified as an E2F-family binding protein that modulates E2F-regulated transcription and may be required for S phase entry. EAPP is expressed at varied levels in all tissues and cell types examined and its expression is reduced in nocodazole-arrested cells (2). Mass spectrometry studies have identified multiple EAPP phosphorylation sites including Ser109 and Ser111, but biological consequences of EAPP phosphorylation have yet to be elucidated (3-5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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