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Product last modified at: 2025-01-01T09:03:26.258Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

EED Antibody #51673

Filter:
  • WB
Western Blotting Image 1: EED Antibody
Western blot analysis of extracts from 293T cells, either transfected with nontargeting siRNA (293T siNT) or siRNA targeting EED (293T siEED), and F9 cells using EED Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 51673

Cat. # Size Qty. Price Ships
51673S 100 µl
$306

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 50-70
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

EED Antibody recognizes endogenous levels of total EED protein. This antibody also cross-reacts with an unidentified protein of 42 kDa.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EED protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Methylation of Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (5). Embryonic ectoderm development protein (EED) is a component of the PRC2 complex, which together with Ezh2 and SUZ12 is absolutely required for histone methyl-transferase activity (6). EED, SUZ12 and EZH2 are overexpressed in various types of cancer, including tumors of the colon, breast, prostate and ovary (7-9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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