Mouse Interleukin-4 (mIL-4) #5208
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Product Name
Inquiry Info. # 5208
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Product Information
Product Usage Information
With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final mIL-4 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of mIL-4 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mIL-4 should be greater than 50 μg/ml.
Formulation
Storage
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 16 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-4. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg mIL-4. |
| Activity | The bioactivity of recombinant mIL-4 was determined in an HT-2 cell proliferation assay. The ED50 of each lot is between 100-400 pg/ml. |
| Molecular Formula | Recombinant mIL-4 contains no "tags" and the nonglycosylated protein has a calculated MW of 13,557. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptide due to glycosylation. The expected amino-terminal HIHGC of recombinant mIL-4 was verified by amino acid sequencing. |
Source / Purification
Background
Limited Uses
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